MIA BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human MIA in cell culture supernates, serum, plasma (heparin, EDTA) and saliva.\n\nMelanoma Inhibiting Activity (MIA), also known as cartilage-derived retinoic acid-sensitive protein (CD-RAP), is an approximately 11-15kD protein that is expressed as a noncovalent homodimer. MIA is structurally related to OTOR/Otoraplin and MIA-2 in a small family of secreted proteins with one SH3 domain (1-3). And the MIA gene was mapped to 19q13.32-q13.33 by fluorescence in situ hybridization. Beside, It is a marker for malignant melanoma. MIA functions as a chemoattractant for mesenchymal stem cells and enhances their BMP-2 and TGF-b3 induced differentiation into chondrocytes . MIA-deficient mice exhibit delayed chondrocyte differentiation but enhanced chondrocyte proliferation and cartilage repair .\n\nAssay Range:\n15.6pg/ml-1000pg/ml\n\nSensitivity:\n< 10 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nQ16674\n\nEntrez Gene ID:\n8190\n\nKit Components:\n96-well plate pre-coated with anti-MIA, Human\nLyophilized recombinant MIA, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143966

Size

96Tests

References

1. Bosserhoff AK, Kaufmann M, Kaluza B, et al. (August 1997). \"Melanoma-inhibiting activity, a novel serum marker for progression of malignant melanoma\". Cancer Res. 57 (15): 3149