MIA, Human, BioAssay(TM) ELISA Kit (CD-RAP)

MIA, also known as cartilage-derived retinoic acid-sensitive protein (CD-RAP), is a secreted protein expressed in cartilaginous tissue during development and in the adult. MIA was identified based on the fact that it is highly expressed in malignant melanomas, but not in normal melanocytes. MIA has been shown to interact with fibronectin, and promotes invasion and metastasis of melanoma cells, perhaps by blocking integrin-fibronectin interactions. \n\nIntended Use:\nFor the quantitative determination of human Melanoma Inhibiting Activity (MIA) concentrations in cell culture supernates, serum, plasma, and saliva.\n\nSensitivity:\n8.46pg/ml\n\nRange:\n~15.6-1000pg/ml\n\nTest Principle:\nThis BioAssay(TM) employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human MIA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any human MIA present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for human MIA is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human MIA bound in the initial step. The color development is stopped and the intensity of the color is measured.\n\nKit Components:\nMicroplate, 1x96wells\nConjugate, 1x21ml\nStandard, 1x10ng\nAssay Diluent, 1x11ml\nCallibrator Diluent, 1x21ml\nWash buffer Concentrate, 1x21ml\nColor Reagent A, 1x12ml\nColor Reagent B, 1x12ml\nStop Solution, 1x6ml\nPlate Sealers, 4x adhesive strips\n\nStorage and Stability:\nStore powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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