MICA BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human MICA in cell culture supernates, cell lysates, tissue homogenates and serum.\n\nMHC class I polypeptide-related sequence A is a protein that in humans is encoded by the MICA gene.1 The MICA gene encodes a 383-amino acid polypeptide with a predicted mass of 43kD.The MICA and MICB genes occur in a 200-kb region spanning the TNFA and TNFB cluster at 6p21.3.2 MICA and the closely related MICB were recognized by intestinal epithelial T cells expressing diverse V-delta-1 gamma/delta TCRs.3 The MICA protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is further distinguished by its unusual exon-intron organization and preferential expression in fibroblasts and epithelial cells. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. MICA and other members of this family may have been selected for specialized functions that are either ancient or derived from those of typical MHC class I genes, in analogy to some of the nonclassic mouse H-2 genes. The standard product used in this kit is recombinant MICA, which is composed of 286 amino acids with the molecular mass of 59kD.\n\nAssay Range:\n62.5pg/ml-4000pg/ml\n\nSensitivity:\n< 10 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nQ29983\n\nEntrez Gene ID:\n4276\n\nKit Components:\n96-well plate pre-coated with anti-MICA, Human\nLyophilized recombinant MICA, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143967

Size

96Tests

References

1. Bahram S, Bresnahan M, Geraghty DE, Spies T (Aug 1994). \"A second lineage of mammalian major histocompatibility complex class I genes\". Proc Natl Acad Sci U S A 91 (14): 6259-63.\n2. Nalabolu, S. R.; Shukla, H.; Nallur, G.; Parimoo, S.; Weissman, S. M. : Genes in a 220-kb region spanning the TNF cluster in human MHC. Genomics 31: 215-222, 1996.\n3. Groh, V.; Steinle, A.; Bauer, S.; Spies, T. : Recognition of stress-induced MHC molecules by intestinal epithelial gamma-delta T cells. Science 279: 1737-1740, 1998.\n