MICB BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human MICB in cell culture supernates, serum and plasma (heparin, EDTA).\n\nMHC class I polypeptide-related sequence B, also called MICB is a protein that in humans is encoded by the MICB gene. The MICB gene is mapped to 6p21.33. This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. It seems to have no role in antigen presentation. This gene acts as a stress-induced self-antigen that is recognized by gamma delta T cells. It is the ligand for the KLRK1/NKG2D receptor and binds to KLRK1 leads to cell lysis.\n\nAssay Range:\n62.5pg/ml-4000pg/ml\n\nSensitivity:\n< 10 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nQ29980\n\nEntrez Gene ID:\n4277\n\nKit Components:\n96-well plate pre-coated with anti-MICB, Human\nLyophilized recombinant MICB, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143968

Size

96Tests

References

1. Girardi, M., Oppenheim, D. E., Steele, C. R., Lewis, J. M., Glusac, E., Filler, R., Hobby, P., Sutton, B., Tigelaar, R. E., Hayday, A. C. Regulation of cutaneous malignancy by gamma-delta T cells. Science 294: 605-609, 2001.\n2. Stern-Ginossar, N., Elefant, N., Zimmermann, A., Wolf, D. G., Saleh, N., Biton, M., Horwitz, E., Prokocimer, Z., Prichard, M., Hahn, G., Goldman-Wohl, D., Greenfield, C., Yagel, S., Hengel, H., Altuvia, Y., Margalit, H., Mandelboim, O. Host immune system gene targeting by a viral miRNA. Science 317: 376-381, 2007.\n