Microcrystal electron diffraction (MicroED) is a novel technique that uses cryo-transmission electron microscopy (Cryo-TEM) for the three-dimensional (3D) reconstruction of organic small molecules and biological macromolecule crystals based on electron diffraction data. As an emerging technology, MicroED employs electrons as the incident beam to acquire MicroED microcrystal electron diffraction data on a Cryo-TEM.
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