Midkine, Recombinant, Human (MK, Neurite Growth Promoting Factor-2, NEGF-2)

Midkine (MK) is the product of a retinoic acid responsive gene, MK, and is a member of a family of heparin binding factors. It contains 121 amino acid residues including 10 conserved cysteine residues, all of which appear to be disulfide linked. Midkine is expressed during embryogenesis, showing an expression pattern that suggests functions in neurogenesis, cell migration, secondary organogenetic induction, and mesoderm-epithelial interaction. The widespread downregulation of MK in the adult human is reverted in a number of cancers, in which polypeptides are able to act as both transforming growth factors and promoters of angiogenesis. Midkine (MK), induces chemotaxis of human neutrophils and was found to trigger mobilization of intracellular calcium of these cells. Midkine induces histamine release from rat peritoneal mast cells with a rapid response in a dosedependent manner. Midkine is also a potent stimulator of collagen and glycosaminoglycan synthesis.\n\nRecombinant Human Midkine is manufactured with N-terminal fusion of HisTag, having a molecular mass of 14.6kD protein and containing 121 amino acid residues of the human Midkine and 10 additional amino acid residues-HisTag\n\nSequence: \nKKKDKVKKGG PGSECAEWAW GPCTPSSKDC GVGFREGTCG AQTQRIRCRV PCNWKKEFGA DCKYKFENWG ACDGGTGTKV RQGTLKKARY NAQCQETIRV TKPCTPKTKA KAKAKKGKGK D\n\nSpecificity: \nThe amino acid sequence of the recombinant human Midkine is 100% homologous to the amino acid sequence of the human Midkine without signal sequence.\n\nApplications: \nSuitable for use in Western blot and ELISA.\nOther applications have not been tested. \n\nForm:\nSupplied as a lyophilized powder from PBS, pH 7.2.\nReconstitute with 200ul PBS.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile buffer. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n

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SPECIFICATIONS

Catalog Number

M3896B

Size

5ug

Form

Supplied as a lyophilized powder in 0.05M phosphate buffer, 0.1M sodium chloride, pH 7.2.

Purity

(same/more than) 95% (SDS-PAGE); Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.

References

1. Zhongguo Shi Yan Xue Ye Xue Za Zhi 2006 Jun;14(3):442-5 2. Cancer Sci 2006 Jun 23 3. Zhonghua Fu Chan Ke Za Zhi 2006 May;41(5):299-301 4. .Life Sci 2006 Mar 21;5. . Lab Invest 2006 Jul;86(7):645-536. J Clin Oncol 2006 Apr;36(4):207-11.