MIF BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human MIF in cell culture supernates, serum and plasma (heparin, EDTA) and human milk.\n\nMacrophage migration inhibitory factor (MIF) is a protein which in humans is encoded by the MIF gene.1, 2 This gene is located to human chromosome 22q11.2.3 It is remarkably small; it has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1 kb.4 This gene encodes a lymphokine that may be involved in cell-mediated immunity, immunoregulation, and inflammation.5 MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein might form a complex in the cytosol near the peripheral plasma membrane, which may possibly indicate a role in integrin signaling pathways. MIF also plays a central role in the toxic response to endotoxemia and possibly septic shock.Macrophage migration inhibitory factor has been reported to interact with COP9 constitutive photomorphogenic homolog subunit 5, CD74, BNIPL, and CXCR4. The standard product used in this kit is recombinant human MIF, consisting of 115 amino acids with the molecular mass of 12.5kD.\n\nAssay Range:\n156pg/ml-10,000pg/ml\n\nSensitivity:\n< 20 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nI4AY87\n\nEntrez Gene ID:\n4282\n\nKit Components:\n96-well plate pre-coated with anti-MIF, Human\nLyophilized recombinant MIF, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

143969

Size

96Tests

References

1. Weiser WY, Temple PA, Witek-Giannotti JS, Remold HG, Clark SC, David JR (October 1989). \"Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor\". Proc. Natl. Acad. Sci. U.S.A. 86 (19): 7522