Migration Inhibitory Factor, Macrophage, Recombinant, Human (Macrophage MIF, Glycosylation-Inhibiting Factor, GIF, GLIF, MMIF)

The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. \n\nSource: \nRecombinant corresponding to aa1-115 of human Macrophage MIF expressed in \nE. coli (NP_002406).\n\nSequence \nMPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC \nSLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA\n\nMolecular Weight: 12kD (115 aa)\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M3895-07L

Size

100ug

Applications

WB

Reactivities

Hum

Form

Supplied as a liquid in 50mM Tris-HCl, pH 8.0, 0.5mM DTT, 10% glycerol.

Purity

~98% by SDS PAGE.

References

1. Weiser WY., et al (1989) Proc Natl Acad Sci. 86: 7522-7526. 2. Bernhagen J., et al (1994) Biochemistry. 33: 14144-14155. 3. Richard B., (1996) FASEB J. 10: 1607-1613.