Milk Fat Globule-EGF Factor 8, Bovine (MFG-E8, MFGM, Sperm Surface Protein SP47, MP47, Lactadherin) (FITC)

Lactadherin is a widely distributed glycoprotein (~ 50kD), which was originally characterized due to its association with milk fat/lipid globule membranes. Synonymous names are PAS-6/7, bovine-associated mucoprotein, BA-46, P47, and MFG-E8. Structural hallmarks of lactadherin are the presence of two epidermal growth factor (EGF) homology domains (with an RGD peptide motif in the second EGF domain), and two C domains sharing homology with the discoidin family of lectin domains including the phospholipid-binding domains of blood clotting factors V and VIII. Lactadherin shows preferential binding to phosphatidylserine (L-form) in a calcium independent manner. \n\nPurified lactadherin functions as an anticoagulant by blocking phosphatidylserine-containing membrane sites for blood coagulation proteins (10). Fluoresence-labeled lactadherin functions as a sensitive probe for exposed phosphatidylserine on nucleated cells and on stimulated platelets (8, 9). Lactadherin will bind to membranes that have phosphatidylserine content below the threshold for annexin V binding.\n\nLocalization: \nMilk (1), also secreted by stimulated macrophages (2), vascular smooth muscle (3), endothelial cells(4)\n\nPlasma Concentration: \nUnknown in normal adults, measurable in women with metastatic breast cancer (5), measurable in newborn calves after milk meal (1).\n\nMolecular Weight: \n52kD and 47kD (glycosylation variants, PAS-6 and PAS-7 respectively)\n\nExtinction Coefficient: \nE1%, 1cm, 280nm = 17.0\n\nStructure: \nSingle chain with two EGF domains and two C domains. The mouse isoform contains a central proline rich region and the human molecule contains a single EGF domain\n\nPercent Carbohydrate: \n6%, 13% for glycosylation variants\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

M3896-90B1

Size

1ml

Form

Supplied as a liquid TBS, pH 7.4, 1% BSA, 0.02% sodium azide. Labled with FITC.

Purity

~95% (SDS-PAGE)

References

1. Butler, J.E., et al 1980. Differentiation 17:31-40, 2. Hanayama, R., et al S. 2002. Nature 417:182-187, 3. Haggqvist, B., et al 1999. Medin: an integral fragment of aortic smooth muscle cell-produced lactadherin forms the most common human amyloid. Proc Natl Acad Sci U S A 96:8669-8674, 4. Silvestre, J.S., et al. 2005. Nat Med 11:499-506, 5. Ceriani, R.L., et al 1982. Proc Natl Acad Sci U S A 79:5420-5424, 6. Hanayama, R., et al 2004. . Science 304:1147-1150, 7. Hanayama, R., and Nagata, S. 2005. I. Proc Natl Acad Sci U S A 102:16886-16891, 8. Shi, J., et al 2006. Cytometry A 69:1193-1201, 9. Dasgupta, S.K., et al 2006. LTransl Res 148:19-25, 10. Shi, J., and Gilbert, G.E. 2003. Blood 101:2628-2636, 11. Hvarregaard et al., 1996. Eur. J. Biochem. 240.