MIP-2 BioAssay(TM) ELISA Kit, Mouse

Sandwich ELISA quantitative detection kit for Mouse MIP-2 in cell culture supernates, serum and plasma (heparin, EDTA).\n\nMIP is a member of the aquaporin family of membrane-bound water channels.1 MIP family proteins are thought to contain 6 TM domains. Sequence analysis suggests that the proteins may have arisen through tandem, intragenic duplication from an ancestral protein that contained 3 TM domains.2 Major intrinsic protein (MIP, also called MP26) is the predominant fiber cell membrane protein of the ocular lens.3 The major intrinsic protein (MIP) of the vertebrate eye lens is the first identified member of a sequence-related family of cell-membrane proteins that appears to have evolved by gene duplication. Several members of the MIP family transport water (aquaporins), glycerol and other small molecules in microbial, plant and animal cells.4 The standard used in this kit is recombinant mouse MIP-2(A28-N100), consisting of 73 amino acids with the molecular mass of 8kD.\n\nAssay Range:\n15.6pg/ml-1000pg/ml\n\nSensitivity:\n< 5 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP10889\n\nEntrez Gene ID:\n20310\n\nKit Components:\n96-well plate pre-coated with anti-MIP-2, Mouse\nLyophilized recombinant MIP-2, Mouse standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143971

Size

96Tests

References

1. Berry, V., Francis, P., Kaushal, S., Moore, A., Bhattacharya, S. Missense mutations in MIP underlie autosomal dominant 'polymorphic' and lamellar cataracts linked to 12q. Nature Genet. 25: 15-17, 2000.\n2. Chrispeels MJ, Agre P (1994). \"Aquaporins: water channel proteins of plant and animal cells\". Trends Biochem. Sci. 19 (10): 421