MIP-3 alpha/CCL20 BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human MIP-3 alpha in cell culture supernates, serum and plasma (heparin, EDTA).\n\nMacrophage Inflammatory Protein 3a (MIP3a), also called Chemokine, cc motif, ligand 20 (CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3a is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3a/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3a and Serine MIP-3a, the two forms of MIP3a, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3a. \n\nAssay Range:\n15.6pg/ml-1000pg/ml\n\nSensitivity:\n< 1 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP78556\n\nEntrez Gene ID:\n6364\n\nKit Components:\n96-well plate pre-coated with anti-MIP-3 alpha/CCL20, Human\nLyophilized recombinant MIP-3 alpha/CCL20, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143972

Size

96Tests

References

1. Nelson, R. T.; Boyd, J.; Gladue, R. P.; Paradis, T.; Thomas, R.; Cunningham, A. C.; Lira, P.; Brissette, W. H.; Hayes, L.; Hames, L. M.; Neote, K. S.; McColl, S. R. : Genomic organization of the CC chemokine MIP-3-alpha/CCL20/LARC/EXODUS/SCYA20, showing gene structure, splice variants, and chromosome localization. Genomics 73: 28-37, 2001.\n2. Rossi, D. L.; Vicari, A. P.; Franz-Bacon, K.; McClanahan, T. K.; Zlotnik, A. : Identification through bioinformatics of two new macrophage proinflammatory human chemokines. J. Immun. 158: 1033-1036, 1997.\n3. Homey, B.; Dieu-Nosjean, M.-C.; Wiesenborn, A.; Massacrier, C.; Pin, J.-J.; Oldham, E.; Catron, D.; Buchanan, M. E.; Muller, A.; de Waal Malefyt, R.; Deng, G.; Orozco, R.; Ruzicka, T.; Lehmann, P.; Lebecque, S.; Caux, C.; Zlotnik, A. : Up-regulation of macrophage inflammatory protein-3-alpha/CCL20 and CC chemokine receptor 6 in psoriasis. J. Immun. 164: 6621-6632, 2000.\n