Mir-10b (MicroRNA 10b) Detection Kit, BioAssay(TM)

Introduction:\nMicroRNAs (miRNAs) are endogenous regulators of gene expression that are encoded in the genomes of plants, animals and viruses, then processed into ~19-23 nt, single-stranded molecules that become incorporated into the RNA-induced silencing complex (RISC). RISC mediates down-regulation of gene expression through translational inhibition, transcript cleavage, or both. There is emerging evidence that some microRNAs can function as oncogenes or tumor suppressors. Recent study showed that microRNA-10b (miR-10b) is highly expressed in metastatic breast cancer cells and positively regulates cell migration and invasion. Designed for use with MicroRNA qRT-PCR primer sets, the miR-10b One-Step qRT-PCR Detection Kit provides a one-step, simple, robust, inexpensive assay for quantitative analysis of mir-10b expression from total RNA samples, or small RNAs enriched samples. MicroRNA qRT-PCR primer sets were uniquely designed and optimized for detecting mature miRNA with high specificity and sensitivity at low PCR annealing temperature. The reverse PCR primer is also served as RT primer for reverse transcription. This kit contains an enzyme mix, a 2X-concentrated qRT-PCR reaction mixture and a miRNA primer set that contain all the reagents (except templates) needed for running qRT-PCR assay. The passive reference dye ROX is included in a separate tube to make this kit adaptable for many real-time QPCR platforms. Total RNA from metastatic breast cancer cell line MDA-MB-231 is supplied with the kit as positive control. \n\nFeatures:\n

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SPECIFICATIONS

Catalog Number

M3949-97

Size

1Kit

References

1. Ma, L., et al., Nature 449: 682-689 (2007). 2. Liu, J., et al., Science 305: 1437-1441 (2004). 3. Tang, G. Trends Biochem. Sci. 30: 106-114 (2005).