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Mitochondria Cytosol Fractionation BioAssay(TM) Kit

Cat no: M3986

Mitochondria Cytosol Fractionation BioAssay(TM) Kit

Mitochondria Cytosol Fractionation BioAssay(TM) Kit provides unique formulations of reagents for effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of various mammalian cells including both apoptotic and non-apoptotic cells. The enriched mitochondrial and cytosolic fractions can be used for studying apoptotic and signal transduction pathways to detect translocation of any factors interested between the two fractions by Western blotting, ELISA, or other assays. The cell fractionation procedure is so simple and easy to perform. No ultracentrifugation is required and no toxic chemicals are involved. \n \nKit Components 100 assays\nM3896A: Mitochondria Extraction Buffer 10ml\nM3896B: 5X Cytosol Extraction Buffer 20ml \n**M3896C: DTT (1M) 110ml\n**M3896D: Protease Inhibitor Cocktail* 1 vial\n\n*(Add 250ul of DMSO before use)\n\nStorage and Stability:\nStore **M3896C and M3896D at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M3986

Size

100Tests

Applications

ELISA, WB

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SUPPLIER INFO

Applications

ELISA

Reactivities

Hum

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Applications

IF

Hosts

Mouse

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Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

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Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

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Applications

ELISA, FC, IHC, WB

Hosts

Mouse

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Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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