Mitochondrial Glycerol-3-phosphate Dehydrogenase, Recombinant, Human, Western Blot Control (Glycerol-3-phosphate Dehydrogenase, Mitochondrial, GPD-M, GPDH-M, mtGPD, GPD2)

Mitochondrial glycerol-3-phosphate dehydrogenase (mGPD) is a Ca2+-sensitive, FAD-binding protein, located on the outer surface of the inner mitochondrial membrane. mGPD catalyses the oxidation of glycerol-3-phosphate to dihydroxyacetone phosphate (DHAP) with concomitant reduction of the enzyme-bound FAD. Two isoforms have been described for mGPD. Isoform 1 comprises 727aa residues, whereas isoform 2 lacks 126aa residues of the N-terminus. Deficiency of mGPD appears to contribute to the impairment of glucose-stimulated insulin release in several animal models of non-insulin dependent diabetes mellitus.\n\nSource: \nRecombinant corresponding to aa43-600 from human mGPD, fused to 6x His-tag at N-terminal, expressed in E. coli.\n\nApplications:\nSuitable for use in Western Blot as a control. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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