Mitochondrial Protein Immunoprecipitation (IP) BioAssay(TM) Kit

Mitochondria are the power house of the cells and play an essential role in energy production. Damage to the mitochondria activates signaling pathways that induce apoptosis. Mitochondria also regulate and mediate transport of the metabolites and ions needed for oxidative phosphorylation and maintenance of membrane potential for ATP synthesis. Mitochondrial dysfunction leads to several disorders like cardiac dysfunction, diabetes, aging and neurological disorder, mainly caused by mutations in mitochondrial DNA or in nuclear genes that code for mitochondrial components. Thus, mitochondria have several different functions in the cell. ? Sample Type: Mitochondria, mitochondrial extract or cell lysate. Intended Use: Applications for this kit include functional assays and enzymatic assays, maintenance of stable mitochondrial complexes, and is compatible with SDS PAGE, 2D gel, Blue-Native gel and Mass spectrometry. Test Principle: The ready to use mitochondria Protein IP Buffer is optimized for immunoprecipitation (IP and co-IP) using mitochondria and mitochondrial extracts. The buffer is a gentle formulation, which maintains the stability of mitochondrial complexes. The Mitochondrial Protein IP BioAssay(TM) kit is provided with different choices of detergents like n-Dodecyl-beta-D-maltoside, Triton X-100 and digitonin to achieve different stringency conditions for protein-protein interaction studies. Triton X-100 is the most commonly used detergent especially for membrane protein solubilization. However, in case of fragile complexes digitonin or n-Dodecyl-beta-D-maltoside is the choice of detergents. Kit Components: Mitochondria Protein IP Buffer, 1x50ml Wash Buffer, 1x50ml Protease Inhibitor Cocktail, lyophilized 10% n-Dodecyl-beta-D-maltoside, 1x1ml 10% Triton X-100, 1x1ml 10% Digitonin, 1x1ml Storage and Stability: Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.