Mitochondrial Protein Immunoprecipitation (IP) BioAssay(TM) Kit

Mitochondria are the power house of the cells and play an essential role in energy production. Damage to the mitochondria activates signaling pathways that induce apoptosis. Mitochondria also regulate and mediate transport of the metabolites and ions needed for oxidative phosphorylation and maintenance of membrane potential for ATP synthesis. Mitochondrial dysfunction leads to several disorders like cardiac dysfunction, diabetes, aging and neurological disorder, mainly caused by mutations in mitochondrial DNA or in nuclear genes that code for mitochondrial components. Thus, mitochondria have several different functions in the cell. ?\nSample Type:\nMitochondria, mitochondrial extract or cell lysate.\n\nIntended Use:\nApplications for this kit include functional assays and enzymatic assays, maintenance of stable mitochondrial complexes, and is compatible with SDS PAGE, 2D gel, Blue-Native gel and Mass spectrometry. \n\nTest Principle:\nThe ready to use mitochondria Protein IP Buffer is optimized for immunoprecipitation (IP and co-IP) using mitochondria and mitochondrial extracts. The buffer is a gentle formulation, which maintains the stability of mitochondrial complexes. The Mitochondrial Protein IP BioAssay(TM) kit is provided with different choices of detergents like n-Dodecyl-beta-D-maltoside, Triton X-100 and digitonin to achieve different stringency conditions for protein-protein interaction studies. Triton X-100 is the most commonly used detergent especially for membrane protein solubilization. However, in case of fragile complexes digitonin or n-Dodecyl-beta-D-maltoside is the choice of detergents.\n\nKit Components:\nMitochondria Protein IP Buffer, 1x50ml\nWash Buffer, 1x50ml\nProtease Inhibitor Cocktail, lyophilized\n10% n-Dodecyl-beta-D-maltoside, 1x1ml\n10% Triton X-100, 1x1ml\n10% Digitonin, 1x1ml\n\nStorage and Stability:\nStore powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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