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MLH1 (MutL protein homolog 1)

Cat no: 030445

MLH1 (MutL protein homolog 1)

DNA-mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. Lack of a functional DNA-mismatch repair pathway is a common characteristic of several different types of human cancers, either due to an MMR gene mutation or promoter-methylation gene silencing. MLH1 is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in hereditary nonpolyposis colon cancer (HNPCC). MLH1 is an integral part of the protein complex responsible for mismatch repair expressed in lymphocytes, heart, colon, breast, lung, spleen, testis, prostate, thyroid and gall bladder, and is methylated in several ovarian tumors. Loss of MLH1 protein expression is associated with a mutated phenotype, microsatellite instability and a predisposition to cancer. In hereditary nonpolyposis colorectal cancer (HNPCC), an autosomal dominant inherited cancer syndrome that signifies a high risk of colorectal and various other types of cancer, the MLH1 gene exhibits a pathogenic mutation. Inactivation of the MLH1 gene causes genome instability and predisposition to cancer. MLH1 also plays a role in meiotic recombination.\n\nApplications:\nWestern Bloting: 1:500-1:2000.\nImmunohistochemistry: 1:200-1:1000.\nImmunofluorescence: 1:200-1:1000.\nELISA: Suggested dilution 1:10000.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

030445

Size

100ul

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, NHP

Form

Ascites fluid, 0.03% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. Int J Cancer. 2007 Aug 1;121(3):555-8.\n2. Autophagy. 2007 Jul-Aug;3(4):368-70.\n3. Fam Cancer. 2008 Jun;7(2):163-172.

Additional Info

Human, Monkey

Alternative Names

FCC2; COCA2; HNPCC

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