MMP-9 BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human MMP-9 in cell culture supernates, serum, plasma (heparin), saliva and urine.\n\nThe 92kD type IV collagenase is also known as 92kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9 (MMP9). The 72- and 92kD type IV collagenases are members of a group of secreted zinc metalloproteases.1 The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion.2 Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture.3 MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells.4 The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema.5 The standard product used in this kit is recombinant human MMP-9 with the molecular mass of 95kD. The detected MMP-9 includes zymogen and active enzyme.\n\nAssay Range:\n156pg/ml-10,000pg/ml\n\nSensitivity:\n< 5 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP14780\n\nEntrez Gene ID:\n4318\n\nKit Components:\n96-well plate pre-coated with anti-MMP-9, Human\nLyophilized recombinant MMP-9, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143983

Size

96Tests

References

1. Collier, I. E.; Bruns, G. A. P.; Goldberg, G. I.; Gerhard, D. S. On the structure and chromosome location of the 72- and 92-kDa human type IV collagenase genes. Genomics 9: 429-434, 1991. \n2. Turner, H. E.; Nagy, Z.; Esiri, M. M.; Harris, A. L.; Wass, J. A. H. Role of matrix metalloproteinase 9 in pituitary tumor behavior. J. Clin. Endocr. Metab. 85: 2931-2935, 2000. \n3. Zhang, B.; Ye, S.; Herrmann, S.-M.; Eriksson, P.; de Maat, M.; Evans, A.; Arveiler, D.; Luc, G.; Cambien, F.; Hamsten, A.; Watkins, H.; Henney, A. M. Functional polymorphism in the regulatory region of gelatinase B gene in relation to severity of coronary atherosclerosis. Circulation 99: 1788-1794, 1999. \n4. Coussens, L. M.; Tinkle, C. L.; Hanahan, D.; Werb, Z. MMP-9 supplied by bone marrow-derived cells contributes to skin carcinogenesis. Cell 103: 481-490, 2000. \n5. Minematsu, N.; Nakamura, H.; Tateno, H.; Nakajima, T.; Yamaguchi, K. Genetic polymorphism in matrix metalloproteinase-9 and pulmonary emphysema. Biochem. Biophys. Res. Commun. 289: 116-119, 2001. \n