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MMP13, CT (Collagenase 3, Matrix Metalloproteinase-13, MMP-13) (APC)

Cat no: M2428-09D-APC

MMP13, CT (Collagenase 3, Matrix Metalloproteinase-13, MMP-13) (APC)

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development,reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.\n\nApplications: \nSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:50-1:1000\nImmunohistochemistry: 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M2428-09D-APC

Size

200ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Allophycocyanin (APC).

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1.Roy-Beaudry, M., et al., Arthritis Rheum. 48(10):2855-2864 (2003). 2.Liacini, A., et al., Exp. Cell Res. 288(1):208-217 (2003). 3.Hantke, B., et al., Biol. Chem. 384(8):1247-1251 (2003). 4.Im, H.J., et al., J. Biol. Chem. 278(28):25386-25394 (2003). 5.Tardif, G., et al., Osteoarthr. Cartil. 11(7):524-537 (2003).

Additional Info

Recognizes human MMP13.

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