Monobromobimane readily reacts with low molecular weight thiols. Bimanes are useful for detecting the distribution of protein thiols in cells before and after chemical reduction of disulfides.Fluorescence: max. Abs. 398nm; e x 10-3: 5.0
Melting Point: 158-160 degrees C
Solubility: Dichloromethane, DMSO
Method for Determining Identity:
Proton NMR Spectroscopic Analysis
TLC Conditions:
SiO2:Dichloromethane: Methanol: 9.5: 0.5. Visualized with UV; Single spot; Rf=0.50.
Storage and Stability:
May be stored at RT for short-term only. Long-term storage is recommended at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.