Monocarboxylate Transporter 2, Human Control Peptide (MCT2)

Control Peptide for M4470-12B (serum) and M4470-12 (affinity purified)\n\nSynthetic 15-aa peptide sequence within the cytoplasmic, C-terminus of Human MCT2.\n\nMonocarboxylate such as lactate and pyruvate play an important role in cellular metabolism. Lactic acid is produced as the end product of glycolysis. Some tissues, such as white skeletal muscle and, red blood cells, use this pathway to generate most of their ATP under normal physiological conditions. All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia. Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain maintain high rate of glycolysis. Failure to export lactic acid leads to accumulation of cellular lactic acid followed by an increase in pH and inhibition of glycolysis. Some tissues, such as brain, heart, and red skeletal muscle, readily oxidize lactic acid, and must import lactic acid into the cells. Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboxylic acid transporters (MCTs). At least 9 MCT-related proteins (MCT1-9) have been identified in mammals that are expressed in a tissue specific manner.\n\nMCT2/MOT2 (mouse 484 aa, rat 489 aa, human 478 aa, chromosome 12q13) is less widely distributed than MCT. It is associated with tissues that demonstrate a high uptake affinity for lactate and pyruvate such as the kidney and liver (for gluconeogenesis) and neurons (for oxidation). It may function in transporting lactate from intestine and erythrocytes. MCT1 is most closely related to MCT1 (~55% identity, whereas homolog with other MCT1-MCT8 isoforms is less (~35-53%). Both N and C-termini are predicted to be cytoplasmic.\n\nApplications:\nSuitable for use in ELISA or antibody blocking to confirm antibody specificity. Not suitable for Western Blot due to low MW (<3kD). Other applications not tested.\n\nRecommended Dilution: \nELISA: \nCoat with 50-100ng Control Peptide/well\nAntibody blocking:\nUse 5-10ug per 1ug of M4470-12 or 1ul of M4470-12B\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M4470-12A

Size

50ug

Applications

ELISA, WB

Form

Supplied as a liquid in PBS, pH 7.2

Purity

Highly purified

References

(1) Jackson VN et al (1997) Biochem. J. 324, 447-453; Dao L et al (1998) J. Biol. Chem. 273, 28959-28965; Koehler-Stec EM et al (1998) Am. J. Physiol. 275, E516-E524; Price NT et al (1998) Biochem. J. 329, 321-328 (review); Halestrap AP and Price NT (1999) Biochem J. 343, 281-299 (review).\n

Additional Info

Recognizes human MCt2. No significant sequence homology exists with other MCTs.