Monocarboxylate Transporter 8, Human Control Peptide (MCT8, XPCT)

Control Peptide for M4470-70A (antiserum) and M4470-70 (affinity purified)\n\nMonocarboxylate such as lactate and pyruvate play an important role in cellular metabolism. Lactic acid is produced as the end product of glycolysis. Some tissues, such as white skeletal muscle and, red blood cells, use this pathway to generate most of their ATP under normal physiological conditions. All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia. Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain maintain high rate of glycolysis. Failure to export lactic acid leads to accumulation of cellular lactic acid followed by an increase in pH and inhibition of glycolysis. Some tissues, such as brain, heart, and red skeletal muscle, readily oxidize lactic acid, and must import lactic acid into the cells. Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboxylic acid transporters (MCTs). At least 9 MCT-related proteins (MCT1-9) have been identified in mammals that are expressed in a tissue specific manner.\n\nMCT8 or XPCT (X-linked PEST Containing Transporter; (mouse, 545 aa, human 613/598 aa, chromosome xq13.2) is highly expressed in liver, heart, and kidney. Please consult the main MCT page for details on the new nomenclature.\n\nApplications:\nSuitable for use ELISA or Antibody Blocking. Because of its low molecular weight (<3kD), it is not suitable for Western Blot. \nOther applications not tested.\n\nRecommended Dilution:\nELISA: 50-100ng of peptide per well.\nAntibody Blocking: 5-10ug per 1ug M4470-70 (affinity purified) or 1ul M4470-70A (antiserum)\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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