Mouse alpha Macroglobulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of mouse alpha Macroglobulin in plasma, and serum. This assay employs a quantitative competitive enzyme immunoassay technique that measures mouse alpha Macroglobulin in less than 3 hours. A polyclonal antibody specific for mouse alpha Macroglobulin has been pre-coated onto a 96-well microplate with removable strips. Alpha Macroglobulin in standards and samples is competed by a biotinylated alpha Macroglobulin sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.