Mouse anti Human ENG monoclonal antibody, clone 277824

Mouse Anti-Human ENG monoclonal antibody for ELISA(Cap). Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins. Endoglin and beta glycan/T beta b RIII are type III receptors for TGF beta b superfamily ligands, sharing 71% aa identity in the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells. Human endoglin cDNA encodes 658 amino acids (aa) including a 25 aa signal sequence, a 561 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain and a 47 aa cytoplasmic domain. An isoform with a 14 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) endoglin. The human endoglin ECD shares 65-72% aa identity with mouse, rat, bovine, porcine and canine endoglin. Endoglin homodimers interact with TGF-beta b 1 and TGF-beta b 3 (but not TGF-beta b 2), but only after binding T beta b RII. Similarly, they interact with Activin A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-IA/ALK-3 or BMPR-IB/ALK-6. BMP-9, however, is reported to bind endoglin directly. Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of endoglin can inhibit TGF-beta b 1 signals but enhance BMP-7 signals in the same myoblast cell line. In endothelial cells, endoglin inhibits T beta b RI/ALK-5, but enhances ALK-1-mediated activation. Deletion of mouse endoglin causes lethal vascular and cardiovascular defects, and human endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I. These abnormalities confirm the essential function of endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization. In preeclampsia of pregnancy, high levels of proteolytically generated soluble endoglin and VEGF R1 (Flt-1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity.

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SPECIFICATIONS

Catalog Number

CABT-L173

Hosts

Mouse

Reactivities

Hum

Conjugates

Unconj

Clone

277824

Target

ENG

Gene Id

2022

Isotype

IgG1

Clonality

Monoclonal

Storage Temp

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 В°C as supplied. 1 month, 2 to 8 В°C under sterile conditions after reconstitution. 6 months, -20 to -70 В°C under sterile conditions after reconstitution.