Mouse Bronchial Smooth Muscle Cells are isolated from normal mouse bronchi tissue. Mouse Bronchial Smooth Muscle Cells are characterized by immunofluorescence with antibodies specific to Smooth muscle alpha-actin. T25 flasks is required for cell adhension to the culture vessels. Grow cells in ECM-coated culture vessels with 5% CO2. Each vial contains at least 1x10^6 cells per ml.