Mouse Bronchiole Endothelial Cells are isolated from normal mouse bronchi tissue. Mouse Bronchiole Endothelial Cells are characterized by immunofluorescence with antibodies specific to vWF and CD31. T25 flasks is required for cell adhension to the culture vessels. Grow cells in ECM-coated culture vessels with 5% CO2. Each vial contains at least 1x10^6 cells per ml.