Mouse Fibronectin ELISA kit is designed for detection of mouse fibronectin in plasma, serum, urine and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures fibronectin in less than 4 hours. A polyclonal antibody specific for fibronectin has been pre-coated onto a microplate. Fibronectin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for fibronectin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added.