Mouse IGF-1 ELISA kit is designed for detection of mouse IGF-1 in plasma, serum and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique, which measures IGF-1 in 5 hours. A polyclonal antibody specific for mouse IGF-1 has been pre-coated onto a microplate. Mouse IGF-1 in standards and samples is sandwiched by the immobilized antibodyand biotinylated polyclonal antibody specific for mouse IGF-1, which is recognized by a streptavidinperoxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.