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Mouse Lewis Lung Tumor Endothelial Cells

Mouse Lewis Lung Tumor Endothelial Cells

Cat no: CSC-C8623W

Supplier: Creative Bioarray
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Mouse Lewis Lung Tumor-Associated Endothelial Cells (MTLUECM) from�Creative Bioarray�were isolated from pathogen-free laboratory nude mice. Mouse Lewis Lung Cancer Cells were subcutaneously injected into the nude mice for 4-6 weeks and the tumor tissues were then harvested for cell culture. MTLUECM were cultured in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in�Creative Bioarray��Culture Complete Growth Medium generally for 14-35 days. Cells were detached from flasks and immediately cryo-preserved in vials. Cells will be delivered in a frozen vial and each vial contains 0.5x10^6�cells/1 ml. The method we use to isolate tumor-associated endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.
Catalogue number: CSC-C8623W
Size: 1.0 mL
Storage temp: Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 �C) until ready for experimental use. Live cell shipment is also available on request. Never can primary cells be kept at -20 �C.

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