Mouse Nephron Progenitor Cells are isolated from embryonic mouse kidneys. These cells are viable for studies that involve the signaling pathways involved in nephrogenesis and stem/progenitor cell niche within the kidney. They are also applicable in studies of potential stem/progenitor cell regulators as they can easily receive small molecule, recombinant proteins, and viruses. Mouse Nephron Progenitor Cells are characterized by immunofluorescence with antibodies specific to PAX-2. T25 flasks is required for cell adhension to the culture vessels. Grow cells in ECM-coated culture vessels with 5% CO2. Each vial contains at least 1x10^6 cells per ml.