Mouse Regulatory B cell (B10) Flow Kit (CD19 FITC/CD5 PE/CD1d Alexa Fluor(R) 647)

Splenic IL-10-producing CD1dhighCD5+ B cells have been designated as regulatory B cells (Breg or B10). These rare IL-10- producing B cells represent only 1%-2% of spleen B cells and 7%-8% of peritoneal B cells in mice but are not normally detectable in blood or lymph nodes. B10 cells are a unique subset of potent regulatory B cells different from B-1a, B-1b, or CD21+CD23- mantle zone B cells. B10 cells produce IL-10 and negatively regulate T cell function. BioLegend's Mouse Regulatory B Cell Flow Kit is composed of fluorochrome conjugated anti-mouse CD19, CD5, and CD1d. It is optimized for detection or isolation of B10 cells by flow cytometry.

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SPECIFICATIONS

Catalog Number

132601

Size

25 tests

Applications

FC

Hosts

Rat

Reactivities

Mouse

Clone

6D5/53-7.3/1B1

Gene Id

12478

Isotype

Rat IgG2a/IgG2a/IgG2b

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 microl per million cells.Materials provided:FITC anti-mouse CD19/PE anti-mouse CD5/Alexa Fluor(R) 647 anti-mouse CD1d, 25 testsFITC Rat IgG2a/PE Rat IgG2a/Alexa Fluor(R) 647 Rat IgG2b isotype controls, 25 testsMaterials not provided:Cell Staining Buffer (Cat. No. 420201)TruStain FcX (Cat. No. 101319)

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

This kit is guaranteed for nine months. Upon receipt, store at 4C, and protected from prolonged exposure to light. Do not freeze.

Alternative Names

B reg

Regulatory Status

RUO