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Mouse Regulatory B cell (B10) Flow Kit (CD19 FITC/CD5 PE/CD1d Alexa Fluor(R) 647)

Mouse Regulatory B cell (B10) Flow Kit (CD19 FITC/CD5 PE/CD1d Alexa Fluor(R) 647)

Cat no: 132601

Supplier: BioLegend
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Splenic IL-10-producing CD1dhighCD5+ B cells have been designated as regulatory B cells (Breg or B10). These rare IL-10- producing B cells represent only 1%-2% of spleen B cells and 7%-8% of peritoneal B cells in mice but are not normally detectable in blood or lymph nodes. B10 cells are a unique subset of potent regulatory B cells different from B-1a, B-1b, or CD21+CD23- mantle zone B cells. B10 cells produce IL-10 and negatively regulate T cell function. BioLegend's Mouse Regulatory B Cell Flow Kit is composed of fluorochrome conjugated anti-mouse CD19, CD5, and CD1d. It is optimized for detection or isolation of B10 cells by flow cytometry.
Catalogue number: 132601
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry
Size: 25 tests
Clone: 6D5/53-7.3/1B1
Gene: 12478
Dilutions: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 microl per million cells.Materials provided:FITC anti-mouse CD19/PE anti-mouse CD5/Alexa Fluor(R) 647 anti-mouse CD1d, 25 testsFITC Rat IgG2a/PE Rat IgG2a/Alexa Fluor(R) 647 Rat IgG2b isotype controls, 25 testsMaterials not provided:Cell Staining Buffer (Cat. No. 420201)TruStain FcX (Cat. No. 101319)
Storage buffer: This kit is guaranteed for nine months. Upon receipt, store at 4C, and protected from prolonged exposure to light. Do not freeze.
Isotype: Rat IgG2a/IgG2a/IgG2b
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: B reg
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