MRP9 (Multidrug Resistance-associated Protein 9, ATP-binding Cassette Sub-family C Member 12, ABCC12, MGC27071)

Multidrug resistance-associated protein 9 is a protein that in humans is encoded by the ABCC12 gene. This gene is a member of the superfamily of ATP-binding cassette (ABC) transporters and the encoded protein contains two ATP-binding domains and 12 transmembrane regions. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies: ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White. This gene is a member of the MRP subfamily which is involved in multi-drug resistance. This gene and another subfamily member are arranged head-to-tail on chromosome 16q12.1. Increased expression of this gene is associated with breast cancer.\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:20-1:50\nImmunohistochemistry (Acetone fixed frozen): 1:20, followed by incubation with RbxRt IgG and a monoclonal rat APAAP complex. Alternatively after incubation with M4688-39A and washing, slides can be incubated with RbxRt IgG (Biotin) and streptavidin conjugated to HRP. \nImmunocytochemistry : 1:20-1:50 on acetone fixed cytospin preparations.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M4688-39A

Size

1ml

Applications

ICC, IHC, WB

Hosts

Rat

Reactivities

Hum

Form

Supplied as a liquid in 0.7% BSA, 0.1% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Multidrug resistance-associated protein 9 (ABCC12) is present in mouse and boar sperm. Ono N, Van der Heijden I, Scheffer GL, Van de Wetering K, Van Deemter E, De Haas M, Boerke A, Gadella BM, De Rooij DG, Neefjes JJ, Groothuis TA, Oomen L, Brocks L, Ishikawa T, Borst P. Biochem J. 2007 Aug 15;406(1):31-40. 2. ABCC10, ABCC11, and ABCC12. Kruh GD, Guo Y, Hopper-Borge E, Belinsky MG, Chen ZS. Pflugers Arch. 2007 Feb;453(5):675-84. Epub 2006 Jul 26. 3. MRP9, an unusual truncated member of the ABC transporter superfamily, is highly expressed in breast cancer. Bera TK, Iavarone C, Kumar V, Lee S, Lee B, Pastan I. Proc Natl Acad Sci U S A. 2002 May 14;99(10):6997-7002.

Additional Info

Recognizes an internal epitope of human MRP9 at ~150kD.

Alternative Names

ATP-binding Cassette, Sub-family C (CFTR/MRP), Member 12