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MSRA, Recombinant, Human, aa24-235, His-Tag (Methionine Sulfoxide Reductase A, Peptide Methionine Sulfoxide Reductase, Peptide-methionine (S)-S-oxide Reductase, Peptide Met(O) Reductase, Protein-methionine-S-oxide Reductase, PMSR)

Cat no: 045444

MSRA, Recombinant, Human, aa24-235, His-Tag (Methionine Sulfoxide Reductase A, Peptide Methionine Sulfoxide Reductase, Peptide-methionine (S)-S-oxide Reductase, Peptide Met(O) Reductase, Protein-methionine-S-oxide Reductase, PMSR)

MSRA (methionine sulfoxide reductase A ) belongs to the MsrA Met sulfoxide reductase family. This enzyme has an important function as a repair enzyme for proteins that have been inactivated by oxidation. It catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. In enzymology, a MSRA is an enzyme that catalyzes the chemical reaction. The 3 substrates of this enzyme are peptide-L-methionine, thioredoxin disulfide, and H2O, whereas its two products are peptide-L-methionine (R)-S-oxide and thioredoxin.\n\nSource:\nRecombinant corresponding to aa24-235 from human MSRA, fused to His-tag at N-terminus, expressed in E.coli.\n\nMolecular Weight:\n~26.2kD (237aa) confirmed by MALDI-TOF\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MGSHMGNSAS NIVSPQEALP GRKEQTPVAA KHHVNGNRTV EPFPEGTQMA VFGMGCFWGA ERKFWVLKGV YSTQVGFAGG YTSNPTYKEV CSEKTGHAEV VRVVYQPEHM SFEELLKVFW ENHDPTQGMR QGNDHGTQYR SAIYPTSAKQ MEAALSSKEN YQKVLSEHGF GPITTDIREG QTFYYAEDYH QQYLSKNPNG YCGLGGTGVS CPVGIKK\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

045444

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 50mM sodium chloride, 10% glycerol.

Purity

~90% (SDS-PAGE)

References

1. Boschi-Muller S, et al. (2005) Biochim. Biophys. Acta. 1703 (2): 231-8. 2. Hansel A., et al. (2005) Biochim. Biophys. Acta 1703:239-247.

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Applications

ELISA

Reactivities

Hum

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Applications

IF

Hosts

Mouse

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Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info
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