Msx2, Control Peptide (Hox8)

Msx2, also known as HOX-8, is a homeobox containing transcription factor which is a member of the MSH family of homeobox proteins. Homeobox genes (HOX genes), encode DNA binding proteins that recognize specific sequences and modulate transcriptional activity (1). Msx-2 is believed to play a role in regulating bone development, particularly in sutures of cranial bone (5). Msx-2 is also expressed in sites of skin appendage formation and it has been proposed that Msx-2 is involved in the growth of skin and skin appendages (6). Msx2 mRNA is found expressed at higher levels in immature tumors, including a yolk sac tumor, seminoma, and chroiocarcinoma, and also in various tumors of epithelial origin (2). Msx2 gene expression is frequently activated in carcinoma-derived cell lines. The gene is inactive in NIH3T3 cells but is transcriptionally activated after transformation by v-Ki-ras suggesting that the Msx2 may play a role in cell transformation (3). Msx2 defects are a cause of craniosynostosis type 2 (CRS2), a disease that is characterized by premature fusion of calvarial sutures that leads to abnormal skull shape (4).\n\nApplications:\nSuitable for use in ELISA and Antibody Blocking. Other applications not tested.\n\nRecommended Dilution:\nAntibody Blocking: \n5-10ug control peptide per 1ug of IgG or 1ul of antiserum\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nLyophilized powder may be stored at 4 degrees C for short-term only. Stable for 12 months at -20 degrees C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.\n\n\n

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SPECIFICATIONS

Catalog Number

M4692-75AP

Size

1mg

Applications

ICC, IHC, WB

Reactivities

Hum

Form

Supplied as a lyophilized powder. No preservative added.

Purity

Highly purified (HPLC, MS)

References

General References:\n1. Simeone, A., Mavilio, F., Bottero, L., Giampaolo, A., Russo, G., Faiella, A., Boncinelli, E., and Peschle, C. (1986) Nature 320: 763-765. 2. Suzuki, M., Tanaka, M., Iwase, T., Naito, Y., Sugimura, H., and Kino, I. (1993) Biochem. Biophys. Res. Commun. 194: 187-193. 3. Takahashi, C., Akiyama, N., Matsuzaki, T., Takai, S., Kitayama, H., and Noda, M. (1996) Oncogene 12: 2137-2146. 4. Liu, Y.H., Kundu, R., Wu, L., Luo, W., Ignelzi, M.A., Snead, M.L., and Maxson, R.E. (1995) Proc. Natl. Acad. Sci. U.S.A. 92: 6137-6141. 5. Dodig, M., Tadic, T., Kronenberg, M.S., Dacic, S., Liu, Y.H., Maxson, R., Rowe, D.W. and Lichtler, A.C. (1999) Dev. Biol. 209: 298-307. 6. Jiang, T.X., Liu, Y.H., Widelitz, R.B., Kundu, R.K., Maxson, R.E. and Chuong, C.M. (1999) J. Invest. Dermatol. 113: 230-237.

Additional Info

Recognizes Msx2. Species Crossreactivity: Human and rodent.