Multi-Parameter Apoptosis Assay Kit

Cayman's Multi-Parameter Apoptosis Assay Kit includes four different reagents for monitoring several events in apoptosis. It employs FITC-conjugated Annexin V as a probe for phosphatidylserine on the outer membrane of apoptotic cells, TMRE as a probe for mitochondrial membrane potential, 7-AAD as an indicator of membrane permeability/cell viability, and Hoechst Dye to demonstrate nuclear morphology. The kit allows phenotypic characterization of different cell death parameters at a single-cell level.

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SPECIFICATIONS

Catalog Number

600330

Form

1 ea

P Type

Assay Kits|Cell Surface Markers

Weight

0

Storage Temp

-20

Shipping Temp

-20

Additional Info

Cellular events in apoptosis include loss of cell membrane asymmetry, cell shrinkage, membrane blebbing, nuclear fragmentation, and chromatin condensation. Cayman's Multi-Parameter Apoptosis Assay Kit includes four different reagents for monitoring several events in apoptosis. It employs FITC-conjugated Annexin V as a probe for phosphatidylserine on the outer membrane of apoptotic cells, TMRE as a probe for mitochondrial membrane potential, 7-AAD as an indicator of membrane permeability/cell viability, and Hoechst Dye to demonstrate nuclear morphology. The kit allows phenotypic characterization of different cell death parameters at a single-cell level. The assay can be adapted to high content screening with appropriate equipment. The reagents provided in the kit are sufficient to run 100 samples when using flow cytometry, or 500 samples when using a 96-well plate format.