Myelin Basic Protein (MBP, Myelin A1 Protein, Myelin Membrane Encephalitogenic Protein, MGC99675)

Myelin basic protein (MBP) is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system and the peripheral nervous system, respectively. It is most abundant in hemopoietic system and contains seven exons distributed over 32-34kb. MBP isolated from MS brain may differ in charge microheterogeneity which would affect antigenic determinants. MBP is mapped to chromosome 18q22-23. Failure in this gene expression would be correlated in the central white matter with extrapyramidal system degeneration signs. Moreover, it is a candidate autoantigen in the disease multiple sclerosis. \n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot (Human, rat): 1ug/ml\nImmunohistochemistry (Paraffin) (Human, rat, mouse): 1ug/ml \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH20. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M9758-03X

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Marty MC., et al. Proc Natl Acad Sci USA 2002 Jun 25; 99(13):8856-61. 2. Streicher R., Stoffel, W. Biol Chem Hoppe Seyler. 1989 May; 370(5):503-10. 3. McLaurin, JA., et al. J Neurol Sci. 1995 Feb; 128(2):205-11. 4. Lester, A., et al. Pediatr Med Chir. 1996 Mar-Apr; 18(2):201-5. 5. Tranquill., LR., et al. J Neurosci Res. 1996 Sep 15; 45(6):820-8.

Additional Info

Recognizes the human Myelin Basic Protein. \nSpecies Crossreactivity: mouse and rat