Myosin Light Chain 2, aa1-166, His-Tag, Recombinant, Human (MYL2, MLC2, CMH10)

Myosin, light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.\n\nSequence of amino acids:\nMGSSHHHHHH SSGLVPRGSH MAPKKAKKRA GGANSNVFSM FEQTQIQEFK EAFTIMDQNR DGFIDKNDLR DTFAALGRVN VKNEEIDEMI KEAPGPINFT VFLTMFGEKL KGADPEETIL NAFKVFDPEG KGVLKADYVR EMLTTQAERF SKEEVDQMFA AFPPDVTGNL DYKNLVHIIT \nHGEEKD\n\nMolecular Weight: 20.9kD (186aa) \n\nEndotoxin: (same/less than)1.0 EU/ug of protein (determined by LAL method)\n\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M9848-03J

Size

100ug

Form

Supplied as a liquid in 20mM Tris pH 8.0, 20% glycerol, 5mM CaCl2.

Purity

(same/less than) 95% by SDS PAGE. Recombinant human MYL2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

References

1. Macera MJ., et al. (1992).Genomics. 13(3):829-31 2. Poetter K., et al. (1996).Nat Genet. 13(1):63-9