Myosin, Skeletal Muscle, heavy chain (MyHC)

Myosin is one of the two major components of muscle tissue responsible for contraction. In muscle cells myosin is arranged as thick filamentous structures that are arranged in parallel to thin filamentous structures composed of actin. Muscle contraction is actuated by the coupling and uncoupling of thin and thick filaments through a chemical linkage mediated by ATP. The coupling and uncoupling is arranged such that the filaments slide in one direction when coupled and in reverse direction when uncoupled or released. Myosin and actin also serve to facilitate motility in non-muscle cells such as in slime molds where shorter yet similar filamentous structures work together. An increasing number of different myosins are being described. Myosin I is a low molecular weight myosin found in protozoa and does not self-assemble. Myosin II is the classic form found in muscle cells that self assemble into thick bundles. Myosin II is a multimeric protein that is composed of two heavy chains and two light chains.\n\nApplications: \nSuitable for use in Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: Reported to detect myosin heavy chain in zebrafish embryos and skeletal muscle sections fixed with 4% paraformaldehyde.\nWestern Blot: 1:1000-1:5000. Detects Myosin Heavy Chain \n(MyHC) in RIPA lysates from rabbit skeletal muscle cells. Rabbit skeletal cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with M9850-15B (1:1000). Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. \nOptimal dilutions to be determined by the researcher.\n\nRecommended Control:\nRIPA lysates from C2C12 cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M9850-15B

Size

200ul

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Dro/Arth, Rabbit

Form

Supplied as a liquid in 0.07M Tris-glycine, pH 7.4, 0.105M sodium chloride, 0.035% sodium azide, 30% glycerol.

P Type

Mab

Purity

Ascites

Isotype

IgG

References

US Biological Application Reference: 1. Huber, M.D., et al., Mol. Cell Biol. 29: 5718

Additional Info

Recognizes human myosin heavy chain, Mr ~220kD. Immunoblotting with muscle cell lysates will often show several close bands from different myosin heavy chain genes, as well as degradation products. Species Crossreactivity: mouse, rat, rabbit, zebrafish and Drosophila.