NADE (p75 NTR-associated Cell Death Executor, Brain Expressed X-linked 3 Protein 3, Protein BEX3, DXS6984E, Nerve Growth Factor Receptor Associated Protein 1, NGFRAP1, Ovarian Granulosa Cell 13.0kD Protein HGR74, Ovarian Granulosa Cell Protein, BEX3, DXS6

The p75 neurotrophin receptor (p75NTR) is a member of the tumor necrosis receptor superfamily and can mediate cell death and cell survival in response to nerve growth factor (NGF). The p75NTR-associated cell death executor (NADE) mediates apoptosis by interacting with the cell death domain of p75NTR following the binding of NGF by p75NTR. Recent studies have shown that NADE also interacts with second mitochondria-derived activator of caspase (Smac). Co-expression of NADE and Smac promotes TRAIL-induced apoptosis and inhibits XIAP-mediated Smac ubiquitization. It has been suggested that it is this interaction between NADE and Smac that allows apoptosis to proceed. Finally, although initially discovered as an mRNA expressed in ovarian granulosa cells, NADE has been suggested to play a role in the neuronal death seen in epileptic brain damage.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman brain cell lysates.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N0009-30B

Size

50ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Gentry JJ, Barker PA, and Cater BD. The p75 neurotrophin receptor: multiple interactors and numerous functions. Pro. Brain Res. 2004; 146:25-39. 2. Mukai J, Hachiya T, Shoji-Hoshino S, et al. NADE, a p75NTR-associated cell death executor, is involved in signal transduction mediated by the common neurotrophin receptor p75NTR. J. Biol. Chem. 2000; 275:17566-70. 3. Rapp G, Freudenstein J, Klaudiny J, et al. Characterization of three abundant mRNAs from human ovarian granulosa cells. DNA Cell Biol. 1990; 9:479-85. 4. YI J-S, Lee, S-K, Sato T-A, et al. Co-induction of p75NTR and the associated death executor NADE in degenerating hippocampal neurons after kainate-induced seizures in the rat. Neurosci. Lett. 2003; 347:126-30. 5. Yoon K, Jang HD, and Lee SY. Direct interaction of Smac with NADE promotes TRAIL-induced apoptosis. Biochim. Biophys. Res. Comm. 2004; 319:649-54.

Additional Info

Recognizes human NADE. Species Crossreactivity: mouse.