Nampt, Recombinant, Human (Visfatin/PBEF, PBEF1, Pre-B Cell Colony Enhancing Factor 1, Nicotinamide Phosphoribosyltransferase)

Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kD adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.\n\nBiological Activity:\nShows adipogenic effects in stimulated differentiating 3T3-L1 cells.\n\nSequence:\nExtracellular human Nampt (visfatin/PBEF) (aa 1-491) is fused at the N-terminus to a FLAG(R)-tag.\n\nStorage and Stability:\nShort-term Storage: +4 degrees C\nLong-term Storage: -20 degrees C\nWorking aliquots are stable for up to 3 months when stored at -20 degrees C.

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SPECIFICATIONS

Catalog Number

045069

Size

10ug

Form

Supplied as a lyophilized powder from a 0.2um-filtered solution in PBS, pH 7.2.

Purity

>95% (SDS-PAGE)

References

Product Reference: \n?Visfatin induces human endothelial VEGF and MMP-2/9 production via MAPK and PI3K/Akt signalling pathways: novel insights into visfatin-induced angiogenesis: R. Adya, et al.; Cardiovasc. Res. 78, 356 (2008) \n?ssDNA Aptamer-base-surface plasmon resonance biosensor for the detection of retinol binding protein 4 for the early diagnosis of type 2 diabetes: S.J. Lee, et al.; Anal. Chem. 80, 2867 (2008) \n?Pre-B cell colony enhancing factor (PBEF)/visfatin induces secretion of MCP-1 in human endothelial cells: Role in visfatin-induced angiogenesis: R. Adya, et al.; Atherosclerosis 205, 113 (2009) \n?Visfatin an Adipocytokine with Proinflammatory and Immunomodulating Properties: A.R. Moschen, et al.; J. Immunol. 178, 1748 (2007) \n?Visfatin activates eNOS via Akt and MAP kinases and improves endothelial cell function and angiogenesis in vitro and in vivo: translational implications for atherosclerosis: F. Lovren, et al.; Am. J. Physiol. Endocrinol. Metab. 296, E1440 (2009)\n?

Alternative Names

Nicotinamide Phosphoribosyltransferase, Pre-B Cell Colony Enhancing Factor 1, PBEF1