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Nectin 1 (Poliovirus Receptor-related Protein, PRR, PVRL1, PRR1, Vec C, CD111, HveC, HigR)

Cat no: N2167

Nectin 1 (Poliovirus Receptor-related Protein, PRR, PVRL1, PRR1, Vec C, CD111, HveC, HigR)

Nectin-1, also called PVRL1, PRR1, Vec C, CD111, HveC and HigR, is an adhesion molecule widely expressed in cell lines of different lineages, including hematopoietic cells, neuronal cells, endothelial and epithelial cells. Nectin-1 belongs to a new family of immunoglobulin-like molecules that includes four members (Nectin-1, -2, -3 and CD155). Nectin-1 gene is localized to human chromosome 11q23-q24, and encodes at least two different transmembrane isoforms. Nectin-1 is a pan alpha herpes virus entry receptor that mediates entry of many HSV1 and HSV-2 strains, bovine Herpes virus1 (BHV-1) and porcine pseudorabies virus (PRV).\n\nApplications:\nSuitable for use in Immunoprecipitation, Western Blot, Flow Cytometry and ELISA. Other applications have not been tested.\n\nRecommended Dilutions:\nImmunoprecipitation (Native): 5-10ug/reaction\nWestern Blot: 0.1-1ug/ml\nFlow Cytometry: 5-10ug/ml\nELISA: 0.1-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N2167

Size

100ug

Applications

ELISA, FC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography

Isotype

IgG1,k

References

1. Cocchi F, et al. J Virol. 72:9992-10002, 1998. 2. Krummenacher C, et al. J Virol 72:7064-7074, 1998. 3. Krummenacher C, et al. J Virol. 74 (23):10863-10872, 2000.

Additional Info

Recognizes human nectin-1 protein at ~75kD. Reactivity has been confirmed with SY5Y human neuroblastoma cells in Western Blot, and M3A cells (CHO cells constitutively expressing human Nectin-1, in Flow Cytometry).

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