Nectin-2 (Poliovirus Receptor-related Protein 2, Herpes Virus Entry Mediator B, Herpes virus Entry Mediator B, HveB, Murine Herpes Virus Entry Protein B, mHveB, Poliovirus Receptor Homolog, CD112, Pvrl2, Mph, Pvr, Pvs) (FITC)

Nectin (originally isolated as poliovirus receptor-related protein (PRR)) is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectin is colocalized with afadin at cadherin-based cell-cell adherence junctions in various tissues and cell lines. The nectin family consist of nectin-1, nectin-2 and nectin-3. Nectin-2 also known as PRR2 or CD112 is a plasma membrane adhesion molecule localized at adherens junctions which is widely expressed in various cell lines including neuronal, endothelial, epithelial and hematopoietic cells. Next to its role in adherens junctions it functions as alphaherpus virus receptor and acts also as intercellular adhesion molecule and pseudorabies virus receptor. Disruption of mouse nectin-2 leads to infertility of male mice.\n\nApplications:\nSuitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry (Frozen): 1:10. PFA or acetone/methanol fixation is recommended\nFlow Cytometry: 1:10\nWestern Blot: 1:10\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

N0571-50C1

Size

100ug

Applications

ELISA, FC, IHC, IP, WB

Hosts

Rat

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Sakisaka, T et al; Different behavior of l-afadin and neurabin-II during the formation and destruction of cell-cell adherens junction. Oncogene 1999, 18: 1609. 2.Takahashi, K et al; Nectin/PRR: An immunoglobulin-like cell adhesion molecule recruited to cadherin- based adherens junctions through interaction with afadin, a PDZ domain-containing protein. J Cell Biol 1999, 145: 539. 3. Kawabe, H et al; Pilt, a novel peripheral membrane protein at tight junctions in epithelial cells. J Biol 2001, 276: 48350.

Additional Info

Recognizes mouse Nectin-2. Species Crossreactivity: human