Nectin 3 (Nectin-3, AA407785, AU016832, AW538082, CD113, Poliovirus Receptor-related Protein 3, PRR3, Pvrl3)

Nectin (originally isolated as poliovirus receptor-related protein (PRR)) is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectin is colocalized with afadin at cadherin-based cell-cell adherence junctions in various tissues and cell lines. The nectin/PRR-family, also described as the alpha herpes virus receptor family consists of four members, nectin 1, 2, 3 and 4. All the members of the nectin family have two or three slice variants. For nectin 3; nectin 3alpha, 3beta and 3gamma isoforms exist. Both nectin 2 and nectin 3 are ubiquitously expressed whereas nectin 1 is abundantly expressed in brain. Nectin 2 or 3 is expressed in cells where cadherin are not expressed, such as blood cells and spermatids. Nectin 3 is a transmembrane protein that is mainly expressed in testis and placental tissues. Nectin 3/PRR3 interacts in vivo with both long and short isoforms of afadin.\n\nApplications:\nSuitable for use in ELISA, Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Not suitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 1:10\nImmunohistochemistry (Frozen): 1:10 Fixation with PFA or acetone/methanol is recommended.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N0571-55D

Size

100ug

Applications

ELISA, FC, IHC, IP, WB

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG2a

References

1. Satoh-Horikawa K, et al. Nectin-3 a new member of immunoglobulin-like cell adhesion molecules that shows homophilic and heterophilic cell-cell adhesion activities. J Biol Chem 2000, 275: 10291. 2. Mueller S, et al. Loss of nectin-2 at Sertoli-spermatid junctions leads to male infertility and correlates with severe spermatozoan head and midpiece malformation, impaired binding to the zona pellucida, and oocyte penetration. Biol Reprod 2003, 69: 1330.

Additional Info

Recognizes mouse Nectin 3. Does not react with human or rat.

Alternative Names

2610301B19Rik, 3000002N23Rik, 4921513D19Rik