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NEK2, Serine/Threonine Kinase, Recombinant, Human, N-terminal His-Tag, Recombinant, Human (NIMA-Related Kinase 2, Never in mitosis A)

Cat no: N0800-03F

NEK2, Serine/Threonine Kinase, Recombinant, Human, N-terminal His-Tag, Recombinant, Human (NIMA-Related Kinase 2, Never in mitosis A)

Human NEK2 (GenBank Accession No. NM_002497), full length with N-terminal HIS6 tag, MW 53kD, expressed in an Baculovirus infected Sf9 cell expression system. \n\nApplication: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.\n\nSource: Baculovirus infected Sf9 cells Formulated in: 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween-20, 50% glycerol, 3mM DTT. \n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.\n\nPurity: (same/more than)80% by SDS-PAGE. \n\nSpecific Activity: 100 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1nmol of myelin basic protein (MBP) per minute at pH 7.4 and 30 degrees C\n\nAssay buffer: 50mM HEPES, pH 7.4, mM MgCl2, 3 mM MnCl2, 1mM DTT, 3uM Na-orthovanadate, 0.1 mM ATP, 4uM NEK2 substrate, and 0.2ug/ml NEK2.

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SPECIFICATIONS

Catalog Number

N0800-03F

Size

10ug

Reactivities

Hum

References

1. Fletcher, L. etal., Biochim. Biophys. Acta 1744 (2), 89-92 (2005).\n2. Fardilha, M. etal., Ann. N.Y. Acad. Sci. 1030, 468-478 (2004).

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