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Nerve Growth Factor, 2.5S, Murine (NGF)

Cat no: N2050-06C1

Nerve Growth Factor, 2.5S, Murine (NGF)

Mouse NGF (2.5S) was isolated from mouse submaxillary glands by method of Mobley et al (1976) and is a form of b-NGF that has identical biological properties. NGF is known to regulate the survival and development of certain sympathetic and sensory neurons. It is a dimer with 2 identical polypeptide chains and dimeric molecular weight of approximately 26,500D. Isolation and purification of NGF from mouse submaxillary glands yields preparations of NGF (2.5S) with identical biological activity but with cleavages at the amino terminus (with the loss of 8 amino acids) and/or at the carboxy-terminus (with the loss of arginine). These preparations are named 2.5 NGF.\n\nApplications: \nSuitable for use in ELISA. Other applications have not been tested. \n\nRecommended Dilutions: \nOptimal dilutions to be determined by the researcher. \n\nMolecular Weight: The homodimer has a molecular weight of ~26KD and the monomer ~13KD as confirmed by SDS PAGE.\n\nBiological activity: \nTested by stimulation of 8 day old chick embryo dorsal root ganglia.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2050-06C1

Size

500ug

Applications

ELISA

Form

Supplied as a sterile filtered lyophilized powder from 0.1M acetic acid under dry N2 with vacuum. Reconstitute with 500ul sterile ddH2O.

Purity

(same/more than) 90% (SDS electrophoresis)

References

1. Mobley WC, Schenker A, Shooter EM (1976) Biochemistry. 15(25):5543-52.

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