Nerve Growth Factor Receptor, p75NTR (Low-affinity NGF receptor, Gp80-LNGFR, p75 ICD, Low affinity neurotrophin receptor p75NTR)

Nerve Growth Factor Receptor, p75NTR, generated by Chandlers et al. p75NTR, was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. MC192 has a potential use as the ligand for gene delivery into p75NTR-expressing rat cells via a receptor-mediated mechanism. \n\nFunction: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Subunit: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1 (By similarity).\n\nApplications:\nSuitable for use in Immunocytochemistry, Flow Cytometry, ELISA and Immunohistochemistry. Not suitable for use in Western Blot or to block Neurotrophin binding. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 20ug/ml\nELISA: 1:5000\nImmunohistochemistry: 1-2ug/mI\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Aliquots are stable for up to 4 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\nNote: We do not recommended adding glycerol as a cryopreservative.

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SPECIFICATIONS

Catalog Number

N2050-40C

Size

50ug

Applications

ELISA, FC, ICC, IHC

Hosts

Mouse

Reactivities

Rat

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added.

P Type

Mab

Purity

Purified by affinity chromatography.

Isotype

IgG2A

References

1. Chandler, C. E. et al (1984) J Biol Chem 259:6882-6889. 2. Lagares A et al (2007) J of neurosci 27(30):7939-7953.

Additional Info

Recognizes the extracellular domain of the neurotrophin receptor p75NTR in rat. MC192 antibody may be used for immunocytochemical localisation of rat cells expressing p75NTR, ELISA and western blot. Does not react with mouse.