Neudesin, Recombinant, Human, aa32-172 (CIR2, NENF, SCIRP10, SPUF, Neuron-derived Neurotrophic Secreted Protein)

Neudesin (neuronпїЅderived neurotrophic secreted protein), gene name NENF (neudesin neurotrophic factor), also called CIR2 (cell immortalizationпїЅrelated 2) or GIG47, is a secreted, 20пїЅ21kD member of the MAPR (membraneпїЅassociated progesterone receptor) subfamily of the cytochrome b5 family of molecules (1, 2). Human Neudesin is synthesized as a 172 amino acid (aa) precursor that contains a 31 aa signal sequence with a 141aa mature region that possesses a cytochrome b5пїЅlike hemeпїЅbinding domain over aa44пїЅ129, and a lysine acetylation site at aa136 (1пїЅ4). The attachment of hemin to its hemeпїЅbinding domain is necessary for its neurotrophic activity, and the binding of heme accounts for 5пїЅ6kD of its circulating molecular weight (3). Mature human Neudesin shares 97%, 94% and 96% aa identity with mouse, rat, and bovine Neudesin, respectively. Neudesin appears to selectively promote cell survival or proliferation and inhibit differentiation in multiple settings. It is expressed by neuronal progenitors and neurons in the central nervous system, and by preadipocytes in white adipose tissue (5, 6). It promotes neuronal differentiation with limited proliferation and serves as a neuron survival factor, but inhibits both astrocyte and adipocyte differentiation (1, 5, 6). Neudesin overпїЅexpression is found in tumors in the human breast, cervix, colon, lung and skin, and in human tumor cell lines that include some lymphomas and leukemias; transfection studies indicate that it may act as an oncogene (4, 7).\n\nSource:\nRecombinant protein corresponding to aa32-172 from human Neudesin, fused at 6-his tag at C-terminal, CHO-derived.\n\nMolecular Weight:\n~19-21kD\n\nEndotoxin:\n<0.10EU/1ug (LAL method)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute in sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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