NeuN (Neuronal Nuclei, Neuron Specific Nuclear Protein) (Biotin)

NeuN is a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: 1:100\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nAdult mouse brain tissue\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

029554

Size

100ul

Applications

IHC

Hosts

Rabbit

Reactivities

Mouse

Conjugates

Biotin

Form

Supplied as a liquid in PBS, 0.1% sodium azide, 15mg/ml BSA. Labeled with Biotin.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Chera, B., et al. (2002). Ann. N.Y. Acad. Sci. 965:274-280. 2. Mullen, et al. (1992). Development. 116:201- 211. 3. Wolf, et al. (1996). J. Histochem. Cytochem. 44:1167-1171. 4. Wood, G. and Warnke, R. (1981). Journal of Histochemistry and Cytochemistry. 29:1196-1204.

Additional Info

Recognizes mouse NeuN.