Neuraminidase, Clostridium perfringens

Neuraminidase (sialidase) splits off N-acetyl neuraminic acid (sialic acid) from a variety of glycoproteins and are important tools for the study of glycoproteins in protein chemistry and cell biology (Hatton et al. 1973). Chromatographic purification has been developed in this laboratory based on work of Cassidy et al. (1965). Wooley and Gommi (1966) describe the use of this neuraminidase in a method for measuring serotonin receptors. It is also employed to determine bound N-acetylneuraminic acid in tissues. Considerable interest has been shown in using neuraminidase inhibitors as possible anti-viral and anti-bacterial agents.\n\nProtein Content (%):\n(same/more than) 30%\n\nUnits/Protein (u/mg):\n(same/more than) 10u/mg Protein\n\nA280@mg/ml:\n~0.4\n\nAssay Method: The assay is based on the measurement of sialic acid (NANA) released from bovine submaxillary mucin. One unit causes the release of one micromole of sialic acid per minute at 37 degrees C and pH 5.0, from bovine submaxillary mucin under the specified conditions.

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SPECIFICATIONS

Catalog Number

N2100

Size

5U

Purity

Supplied as a lyophilized powder, 50% sucrose.

References

1. Aalto, M., et al., Biochim. Biophys. Acta 342: 247 (1974). 2. Burton, R., J. Neurochem. 10: 503 (1963). 3. Cassidy, J., et al., J. Biol. Chem. 240: 3501 (1965). 4. Drzeniek, R., Nature 211: 1205 (1966). 5. Drzeniek, R. & Gauhe, A., Biochem. Biophys. Res. Comm. 38: 651 (1970). 6. Galili, U. & Schlesinger, M., J. Immunol. 112: 1628 (1974). 7. Gasic, H. & Gasic, T., Proc. Natl. Acad. Sci. USA 67: 793 (1970). 9. Groome, N. & Belyavin, G., Anal. Biochem. 63: 249 (1975). 10. Hatton, M. & Regoczi, E., Biochim. Biophys. Acta 327: 114 (1973). 11. Wooley, D. & Gommi, B., Arch. Intl. Pharmocodyn. 159: 8 (1966).\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\nHaskell, T., Peterson, F., Watson, D. Plessas, N., and Culbertson, T.: Neuraminidase Inhibition and Viral Chemotherapy, J. Med. Chem., 13, 697 (1970).\n\nHatton, M., and Regoczi, E.: A Simple Method for the Purification of Commercial Neuraminidase Preparations Free from Proteases, Biochim. Biophys. Acta, 327, 114 (1973).\n\nHohnquist, L., and Brossmer, R.: On the Specificity of Neuraminidase. The Carboxylmethyl [[alpha]]-Ketoside of N-Acetyl-D-Neuraminic Acid, A Vibrio cholerae Neuraminidase Substrate Having Two Anionic Sites, FEBS Lett., 22, 46 (1972).\n\nHowe, C., and Schuluederberg, A.: Neuraminidase Associated with Measles Virus, Biochem. Biophys. Res. Comm., 40, 606 (1970).\n\nInouye, K., and Shigeta, K.: Effects of Ethanol on the Activity of Clostridium-Perfringens Sialidase

Alternative Names

EC=3.2.1.18; Clostridium perfringens