Neurexin 1 alpha, Recombinant, Rat (NRXN1A)

Neurexin 1, also known as NRXN1A, is a neuronal type I transmembrane molecule that is encoded by one of three Neurexin genes (1, 2). Mature rat Neurexin 1 is a 130-200kD N- and O-glycosylated protein with a 1424 amino acid (aa) extracellular domain (ECD) and a 55 aa cytoplasmic region. The ECD contains six LNS domains interspersed with three EGF-like domains and a juxtamembrane O-glycosylation region; the cytoplasmic domain contains a PDZ recognition motif (1, 2). The ECD of rat Neurexin 1 shares 92% and 97% aa sequence identity with human and mouse Neurexin 1 , respectively. It shares 68% aa sequence identity with rat Neurexin 2 and 3 . Transcription of the Neurexin 1 gene from an internal promoter gives rise to Neurexin 1 which lacks the first five LNS domains and all of the EGF-like domains (3). Neurexins are combinatorially spliced at five canonical sites, giving rise to over 1000 potential isoforms (3, 4). This recombinant protein corresponds to the ECD of SwissProt #Q63372, isoform 13, which has short deletions at splice sites 1 and 2. Neurexin 1 is primarily expressed in the brain where it is localized to presynaptic terminals (3, 5). It binds black widow spider toxin -latrotoxin, plus dystroglycan, neurexophilin 1 and 3, and the postsynaptic neuroligins (6-9). The interaction between Neurexins and neuroligins is complex and is restricted to particular combinations of splice forms of each binding partner (9). These interactions promote the differentiation and maintenance of postsynaptic GABA and NMDA, but not AMPA terminals (10, 11). At presynaptic terminals, as well as in pituitary melanotrophs, alpha Neurexins are required for coupling voltage-dependent calcium channel signaling to neurotransmitter or hormone release (12-14).\n\nN-terminal Sequence Analysis: \nLeu31\n\nSDS-PAGE: \n165-175kD, reducing conditions.\n\nActivity: \nMeasured by its binding ability in a functional ELISA. \n\nSpecific Activity:\nImmobilized rrNRXN-1a at 0.5ug/ml (100ul/well) can bind biotinylated rrNXPH-1 with a linear range of 0.03-2ug/ml.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2110-05

Size

50ug

Reactivities

Rat

Form

Supplied as a lyophilized powder in PBS. Reconstitute with 500ul sterile PBS.

Purity

(same/more than) 80%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin (same/less than)1EU/ug (LAL).

References

1. Craig, A.M. and Kang, Y., 2007, Curr. Opin. Neurobiol. 17:43. 8. Missler, M. et al., 1998, J. Biol. Chem. 273:34716.\n2. Lise, M.-F. and El-Husseini, A., 2006, Cell. Mol. Life Sci. 63:1833. 9. Boucard, A.A. et al., 2005, Neuron 48:229.\n3. Ushkaryov, Y.A. et al., 1992, Science 257:50. 10. Chih, B. et al., 2006, Neuron 51:171.\n4. Rowen, L. et al., 2002, Genomics 79:587. 11. Kattenstroth, G. et al., 2004, Proc. Natl. Acad. Sci. 101:2607.\n5. Dean, C. et al., 2003, Nat. Neurosci. 7:708. 12. Missler, M. et al., 2003, Nature 423:939.\n6. Geppert, M. et al., 1998, J. Biol. Chem. 273:1705. 13. Zhang, W. et al., 2005, J. Neurosci. 25:4330.\n7. Sugita, S. et al., 2001, J. Cell Biol. 154:435. 14. Dudanova, I. et al., 2006, J. Neurosci. 26:10599.