Neurite Outgrowth Inhibitor Protein A (NogoA)

NogoA is a member of a family of integral membrane proteins termed reticulons that are thought to be involved in numerous disorders including neurodegenerative diseases. Reticulon proteins are known to regulate many cellular processes and interact with multiple proteins and receptors such as BACE (reviewed in 1). NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor (2). It is highly expressed in oligodendrocytes in the white matter of\nthe CNS; blocking its activity with antibodies or other factors results in improved axon regrowth and functional recovery in experimental CNS lesion models (3). NogoA has also been suggested to play a role in neurodegenerative diseases such as Amyotrophic lateral sclerosis, in which case NogoA is found at elevated levels in postmortem muscular samples (4), and multiple sclerosis (MS), in which case autoantibodies to NogoA have been found in serum and cerebrospinal fluid in MS patients (5). Despite its predicted molecular weight, NogoA typically migrates at 180kDa in an SDS-PAGE. At least five isoforms of Nogo are known to exist; this antibody is specific for NogoA.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-1 ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMouse brain tissue lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2116-45

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Yan R, ShiQ, Hu X, et al. Reticulon proteins: emerging players in neurodegenerative diseases. Cell. Mol. Life Sci. 2006; 63:877-889. 2. Chen MS, Huber AB, van der Haar ME, et al. Nogo-A is a myelin-associated neurite outgrowth inhibitor and an antigen for monoclonal antibody IN-1. Nature 2000; 403:434-9. 3. Schweigreiter R and Bandtlow CE. Nogo in the injured spinal cord. J. Neurotrauma 2006; 3-4:384-96. 4. Dupuis L, Gonzalez de Aguilar JL, di Scala F, et al. Nogo provides a molecular marker for diagnosis of amyloid lateral sclerosis. Neurobiol. Dis. 2002; 10:358-65. 5. Reinl M, Khantane S, Ehling R, et al. Serum and cerebrospinal fluid antibodies to Nogo-A in patients with multiple sclerosis and acute neurological disorders. J. Neuroimmunol. 2003; 145:139-47.

Additional Info

Recognizes human NogoA. Species Crossreactivity: Mouse and rat.